Molecular study of porcine transmissible gastroenteritis virus after serial animal passages revealed point mutations in S protein.
Identifieur interne : 002078 ( Main/Exploration ); précédent : 002077; suivant : 002079Molecular study of porcine transmissible gastroenteritis virus after serial animal passages revealed point mutations in S protein.
Auteurs : Nabil Ben Salem Abid [Russie] ; Sergei A. Chupin ; Olga P. Bjadovskaya ; Olga G. Andreeva ; Mahjoub Aouni ; Javier Buesa ; Taufik Z. Baybikov ; Larisa B. ProkhvatilovaSource :
- Virus genes [ 1572-994X ] ; 2011.
Descripteurs français
- KwdFr :
- ARN viral, Analyse de séquence d'ARN, Animaux, Cellules cultivées, Données de séquences moléculaires, Gastroentérite transmissible du porc (virologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (biosynthèse), Glycoprotéines membranaires (génétique), Mutation ponctuelle, Protéines de l'enveloppe virale (biosynthèse), Protéines de l'enveloppe virale (génétique), Suidae (virologie), Séquence d'acides aminés, Virus de la gastroentérite transmissible (génétique).
- MESH :
- biosynthèse : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus de la gastroentérite transmissible.
- virologie : Gastroentérite transmissible du porc, Suidae.
- ARN viral, Analyse de séquence d'ARN, Animaux, Cellules cultivées, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Mutation ponctuelle, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Cells, Cultured, Gastroenteritis, Transmissible, of Swine (virology), Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (genetics), Molecular Sequence Data, Point Mutation, RNA, Viral, Sequence Analysis, RNA, Spike Glycoprotein, Coronavirus, Swine (virology), Transmissible gastroenteritis virus (genetics), Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (genetics).
- MESH :
- chemical , biosynthesis : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Transmissible gastroenteritis virus.
- virology : Gastroenteritis, Transmissible, of Swine, Swine.
- Amino Acid Sequence, Animals, Cells, Cultured, Molecular Sequence Data, Point Mutation, RNA, Viral, Sequence Analysis, RNA, Spike Glycoprotein, Coronavirus.
Abstract
Porcine respiratory coronavirus is related genetically to porcine transmissible gastroenteritis virus with a large deletion in S protein. The respiratory virus is a mutated form that may be a consequence of the gastroenteritis virus's evolution. Intensive passages of the virus in its natural host may enhance the appearance of mutations and therefore may contribute to any attenuated form of the virus. The objective of this study was to characterize the porcine transmissible gastroenteritis virus TMK22 strain after passages in piglets from 1992 until 2007. A typical experimental infection, molecular characterization, and serological analysis were also carried out to further characterize and to evaluate any significant difference between strains. The sequence analysis showed two amino acid deletions and loss of an N-glycosylation site in transmissible gastroenteritis virus S protein after passages in piglets. Although these deletions were positioned at the beginning of the antigenic site B of S protein, no clinical differences were observed in piglets infected experimentally either with the native virus or the mutated one. Serological tests did not show any antibody reactivity difference between the two strains. In this article, we report that the S protein deletion did not affect the virus's pathogenicity. The variety of the virus's evolutionary forms may be a result, not only of the multiple passages in natural hosts, but also of other factors, such as different pathogens co-infection, nutrition, immunity, and others. Further studies need to be carried out to characterize the mutated strain.
DOI: 10.1007/s11262-010-0562-8
PubMed: 21188626
Affiliations:
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Chupin</name></author><author><name sortKey="Bjadovskaya, Olga P" sort="Bjadovskaya, Olga P" uniqKey="Bjadovskaya O" first="Olga P" last="Bjadovskaya">Olga P. Bjadovskaya</name></author><author><name sortKey="Andreeva, Olga G" sort="Andreeva, Olga G" uniqKey="Andreeva O" first="Olga G" last="Andreeva">Olga G. Andreeva</name></author><author><name sortKey="Aouni, Mahjoub" sort="Aouni, Mahjoub" uniqKey="Aouni M" first="Mahjoub" last="Aouni">Mahjoub Aouni</name></author><author><name sortKey="Buesa, Javier" sort="Buesa, Javier" uniqKey="Buesa J" first="Javier" last="Buesa">Javier Buesa</name></author><author><name sortKey="Baybikov, Taufik Z" sort="Baybikov, Taufik Z" uniqKey="Baybikov T" first="Taufik Z" last="Baybikov">Taufik Z. Baybikov</name></author><author><name sortKey="Prokhvatilova, Larisa B" sort="Prokhvatilova, Larisa B" uniqKey="Prokhvatilova L" first="Larisa B" last="Prokhvatilova">Larisa B. Prokhvatilova</name></author></analytic><series><title level="j">Virus genes</title><idno type="eISSN">1572-994X</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cells, Cultured</term><term>Gastroenteritis, Transmissible, of Swine (virology)</term><term>Membrane Glycoproteins (biosynthesis)</term><term>Membrane Glycoproteins (genetics)</term><term>Molecular Sequence Data</term><term>Point Mutation</term><term>RNA, Viral</term><term>Sequence Analysis, RNA</term><term>Spike Glycoprotein, Coronavirus</term><term>Swine (virology)</term><term>Transmissible gastroenteritis virus (genetics)</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral</term><term>Analyse de séquence d'ARN</term><term>Animaux</term><term>Cellules cultivées</term><term>Données de séquences moléculaires</term><term>Gastroentérite transmissible du porc (virologie)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (biosynthèse)</term><term>Glycoprotéines membranaires (génétique)</term><term>Mutation ponctuelle</term><term>Protéines de l'enveloppe virale (biosynthèse)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Suidae (virologie)</term><term>Séquence d'acides aminés</term><term>Virus de la gastroentérite transmissible (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Transmissible gastroenteritis virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Virus de la gastroentérite transmissible</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Gastroentérite transmissible du porc</term><term>Suidae</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Gastroenteritis, Transmissible, of Swine</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cells, Cultured</term><term>Molecular Sequence Data</term><term>Point Mutation</term><term>RNA, Viral</term><term>Sequence Analysis, RNA</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN viral</term><term>Analyse de séquence d'ARN</term><term>Animaux</term><term>Cellules cultivées</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Mutation ponctuelle</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Porcine respiratory coronavirus is related genetically to porcine transmissible gastroenteritis virus with a large deletion in S protein. The respiratory virus is a mutated form that may be a consequence of the gastroenteritis virus's evolution. Intensive passages of the virus in its natural host may enhance the appearance of mutations and therefore may contribute to any attenuated form of the virus. The objective of this study was to characterize the porcine transmissible gastroenteritis virus TMK22 strain after passages in piglets from 1992 until 2007. A typical experimental infection, molecular characterization, and serological analysis were also carried out to further characterize and to evaluate any significant difference between strains. The sequence analysis showed two amino acid deletions and loss of an N-glycosylation site in transmissible gastroenteritis virus S protein after passages in piglets. Although these deletions were positioned at the beginning of the antigenic site B of S protein, no clinical differences were observed in piglets infected experimentally either with the native virus or the mutated one. Serological tests did not show any antibody reactivity difference between the two strains. In this article, we report that the S protein deletion did not affect the virus's pathogenicity. The variety of the virus's evolutionary forms may be a result, not only of the multiple passages in natural hosts, but also of other factors, such as different pathogens co-infection, nutrition, immunity, and others. Further studies need to be carried out to characterize the mutated strain.</div></front></TEI><affiliations><list><country><li>Russie</li></country></list><tree><noCountry><name sortKey="Andreeva, Olga G" sort="Andreeva, Olga G" uniqKey="Andreeva O" first="Olga G" last="Andreeva">Olga G. Andreeva</name><name sortKey="Aouni, Mahjoub" sort="Aouni, Mahjoub" uniqKey="Aouni M" first="Mahjoub" last="Aouni">Mahjoub Aouni</name><name sortKey="Baybikov, Taufik Z" sort="Baybikov, Taufik Z" uniqKey="Baybikov T" first="Taufik Z" last="Baybikov">Taufik Z. Baybikov</name><name sortKey="Bjadovskaya, Olga P" sort="Bjadovskaya, Olga P" uniqKey="Bjadovskaya O" first="Olga P" last="Bjadovskaya">Olga P. Bjadovskaya</name><name sortKey="Buesa, Javier" sort="Buesa, Javier" uniqKey="Buesa J" first="Javier" last="Buesa">Javier Buesa</name><name sortKey="Chupin, Sergei A" sort="Chupin, Sergei A" uniqKey="Chupin S" first="Sergei A" last="Chupin">Sergei A. Chupin</name><name sortKey="Prokhvatilova, Larisa B" sort="Prokhvatilova, Larisa B" uniqKey="Prokhvatilova L" first="Larisa B" last="Prokhvatilova">Larisa B. Prokhvatilova</name></noCountry><country name="Russie"><noRegion><name sortKey="Abid, Nabil Ben Salem" sort="Abid, Nabil Ben Salem" uniqKey="Abid N" first="Nabil Ben Salem" last="Abid">Nabil Ben Salem Abid</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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